How long is a piece of string! Could be engine oil, pump.... could be rad, fans... could be rad cap... water pump, pulley / pump drive... could be many things. VQs run hot, has this suddenly started or slowly got worse? Have you recently done any mods or jobs when this started?
And silly question but have you checked it is actually overheating and not faulty sender etc?
You didn't say if your heater gets hot, when you filled the system and burping it. You want it on full, and if it's not getting hot you have an air problem.
Thermostats you can check in a boiling saucepan and digital thermometer.
Probably if it were me and based on what you've done so far (and assuming the engine has oil) I'd stick a temp probe on to confirm it's overheating and not a sender / gauge fault, and if so loosen the drive belt and give the water pump pulley a wiggle, then stick a pressure gauge inline to test water pump impeller.